TABLE 3.

Substrate specificity of the GST from Rhodococcus sp. strain AD45

Substrate	% of activitya
Isoprene monoxide	100
Epoxyethane	37
Epoxypropane	32
1,2-Epoxybutane	26
1,2-Epoxyhexane	25
cis-2,3-Epoxybutane	13
Epithiopropane	34
Epifluorohydrin	28
Epichlorohydrin	31
Epibromohydrin	25
cis-1,2-Dichloroepoxyethane	4

^aAll activities were determined in 50 mM Tris-HCl buffer (pH 8.5) with 5 mM GSH except for epifluorohydrin, epichlorohydrin, and epibromohydrin, in which case 50 mM Tris-HCl (pH 8.0) was used to reduce the chemical reaction rate between these substrates and GSH. Activities are expressed as percentages of the activity found, at the same pH, with isoprene monoxide.