TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− λ−recA1 Δ(lacZYA-argF)U169 hsdR17 thi-1 gyrA96 supE44 endA1 relA1 φ80dlacZΔM15 | GIBCO-BRL |
| S17-1 λpir | S17-1 lysogenized with λpir phage | 33 |
| R. palustris | ||
| CGA009 | Wild-type strain; spontaneous Cmr derivative | 16 |
| CGA606 | badE::′lacZ; Kmr | 8 |
| CGA607 | badE::′lacZ aadR; Kmr | This study |
| CGA609 | badR; Gmr | This study |
| CGA610 | badE::′lacZ badR; Kmr Gmr | This study |
| CGA611 | aadR badE::′lacZ badR; Kmr Gmr | This study |
| CGA615 | aadR badR; Gmr | This study |
| CGA101 | aadR | 6 |
| Plasmids | ||
| pUC19 | High-copy-number cloning vector; Apr | 38 |
| pCF116 | Mobilizable suicide vector; sacB; Kmr | 9 |
| pGMΩ1 | Contains the Gmr cassette | 32 |
| pPE408 | pJQ200KS; sacB suicide vector containing badE::′lacZ | 8 |
| pPE600 | pUC19 with a 1,873-bp fragment containing badR; Apr | This study |
| pPE601 | pPE600 with a Gmr cassette at the MscI site in badR | This study |
| pPE602 | pCF116 containing badR::Gmr inserted at SacI-XbaI sites | This study |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Gmr, gentamicin resistance; Cmr, chloramphenicol resistance.