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. 2022 Aug 5;17(10):2307–2325. doi: 10.1038/s41596-022-00727-1

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Fig. 2 — Various types of samples can be processed by this protocol using two workflows. Workflow A comprises four major steps, including tissue lysis, protein reduction and alkylation, Lys-C digestion and trypsin digestion. A simplified workflow B can achieve comparable performance. The resultant peptides are cleaned using C18 cartridges before LC–MS/MS analysis. NPT, normal pressure and temperature; ESI, electrospray ionization; TOF-MS, time-of-flight mass spectrometry.