PCT scheme	Parameters	Setting
Lysis	Pressure	45 kpsi
	Cycles	90 (60 for cell samples)
	On time	25 s
	Off time	10 s
	Temperature	30 °C
Lys-C digestion	Pressure	20 kpsi
	Cycles	45
	On time	50 s
	Off time	10 s
	Temperature	30 °C
Trypsin digestion	Pressure	20 kpsi
	Cycles	90
	On time	50 s
	Off time	10 s
	Temperature	30 °C