Fig. 5.

Effect of TFP on DENV-2 RNA and protein expression. (a) DENV-2-infected Vero cells were treated (TFP) or not treated (VC) with 30 µM TFP. At 24 h p.i., total RNA was extracted from the cells to determine the amount of viral RNA by qRT-PCR. Results are expressed as viral RNA copies, and data are mean values from three independent experiments ± SD. Statistical significance was evaluated using an unpaired t-test (*, p < 0.05). (b) Vero cells treated (TFP) or not treated (VC) with TFP as in panel a were fixed and processed for immunofluorescence to detect the E glycoprotein. Cell nuclei were stained with Hoechst 33258. Magnification, 600X. (c) DENV-2-infected Vero cells were treated (TFP) or not treated (VC) with 30 µM TFP, and at 24 h p.i. the expression of NS5 protein was analyzed by Western blot. GAPDH was used as a loading control. CC, control uninfected cells