Extended Data Fig. 4. The identification and classic functions of platelets from platelet-specific Gsdmd KO mice.
a, b, The Gsdmdfl/fl PF4-Cre mice were identified by PCR (a) and confirmed by western blot (b), respectively (n = 6). c, The platelet (isolated from mice) suspensions were incubated with 0.1 U/ml thrombin for 30 minutes. P selectin translocation to membrane was assessed by FACS after stimulation with thrombin. The representative plots were presented as the number of counts over the log of associated fluorescence (baseline refers to the group without thrombin). Quantification of data presented as percentage of platelet activation. Data are expressed as mean ± SD (Gsdmdfl/fl + HBSS, n = 5; Gsdmdfl/fl + Thrombin, n = 6; Gsdmdfl/fl PF4-Cre+Thrombin, n = 4). d, Tail bleeding times of mouse was measured with the tail dipped into warmed saline to assess haemostasis using a tail-guillotine. Data are expressed as mean ± SD (Gsdmdfl/fl mice, n = 7; Gsdmdfl/fl PF4-Cre mice, n = 6). One-way ANOVA and Tukey’s multiple comparisons test for c. Unpaired t test with two-tailed for d. MT, mutation; WT, wild type; Tg, transgene; Ctrl, control; GSDMD, Gasdermin D; PLTs, platelets; HBSS, hank’s balanced salt solution.