Extended Data Fig. 5. The levels of S100A8/A9 in sepsis patients/mice and the caspase 1 activity in platelets from the CLP or rmS100A8/A9-injected mice.
a-c, Boxplots displaying the level of heterodimer S100A8/A9 in plasma from (a) severe sepsis (with or without septic shock) patients (HS: n = 53, Severe sepsis: n = 51), (b) CLP-induced sepsis mice (Sham, n = 10, CLP: n = 20) and (c) LPS-induced sepsis mice (PBS, n = 8, LPS: n = 8) by ELISA. The boxes indicate the 25% quantile, median, and 75% quantile. d, In a mouse model, mice that were injected intravenously with rmS100A8/A9 (30 μg/kg) or normal saline (n = 4 mouse/group) for 6 hours. Another mouse model, mice were induced CLP for 6 hours. FACS analysis displaying the caspase 1 activity in platelets. Mann Whitney test with two-tailed for a-b. Unpaired t test with two-tailed for c. One-way ANOVA and Tukey’s multiple comparisons test for d. Data was presented as mean ± SD. HS, healthy subjects; Severe sepsis, severe sepsis/septic shock; Sham, sham-operated mice; CLP, CLP-induced sepsis mice; PBS, PBS-injected mice; LPS, LPS-injected mice.