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. 2022 Aug 4;1(8):732–747. doi: 10.1038/s44161-022-00108-7

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© The Author(s), under exclusive licence to Springer Nature Limited 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Extended Data Fig. 8 — a, In platelets from severe sepsis (with or without septic shock) patients, bar graphs displaying change of mitochondrial membrane potential (∆Ψ_m) by staining with 40 nM TMRM using FACS analysis (HS: n = 20, Severe sepsis: n = 25). b, c, In vitro, bar graphs displaying change of mitochondrial ∆Ψ_m (b) and ROS production (c) in platelets (Tlr4^-/- or WT) treated with 1 μg/ml rmS100A8/A9 for 4 hours using FACS analysis (n = 5). d, e, In the LPS induced murine model, mice with platelets depletion were transfused with a total of 1.2 ×10⁷ purified platelets (volume: 200 μl, concentration: 6 ×10¹⁰ platelets/L) from Tlr4^-/- or WT mice (n = 6/group). After 6 hours, bar graphs displaying change of mitochondrial ∆Ψ_m (d) and ROS production (e) in platelets (Tlr4^-/–or WT) using FACS analysis (n = 6). Data was presented as mean fluorescence ± SD. Unpaired t test with two-tailed for a. One-way ANOVA and Tukey’s multiple comparisons test for b-e. Abbreviation is as follow: HS, healthy subjects; Severe sepsis, severe sepsis/septic shock; HBSS, hank’s balanced salt Solution. PBS, PBS-injected mice; LPS + saline, LPS-injected mice transfused with normal saline; LPS + WT PLTs, LPS-injected mice transfused with WT platelets; LPS + Tlr4^-/- PLTs, LPS-injected mice transfused with Tlr4^-/- platelets; TMRM, tetramethylrhodamine methyl ester; ROS, reactive oxygen species.

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