Extended Data Fig. 1. Identified purity of platelet, representative proteins related to different cell death signal pathways and platelet pyroptosis in sepsis.
a, Purified platelets were obtained from human. Purity of platelet preparation was determined by FACS analysis using FITC anti-human CD41a and PE anti-human CD45 (n = 3). b, Heatmap of representative proteins expression related to different cell deaths signal pathways in purified platelet samples from HS (n = 3) and severe sepsis (with or without septic shock) (n = 3) using high-throughput proteomics analysis. c, Bar graphs displaying the percentage of activations of caspase 1 in platelets from sepsis and severe sepsis (with or without septic shock) and HS using FACS (HS: n = 13, Sepsis: n = 10, Severe sepsis with or without septic shock: n = 13). Data was presented as mean ± s.d. Kruskall-Wallis test and Dunn’s multiple comparisons test for c. HS, healthy subjects; Severe sepsis, severe sepsis/septic shock; PLTs, platelets.