K D values and lifetimes (τ) obtained by EF to metal TRLFS titration experiments. The number of coordinated water molecules (q) was calculated for Eu(iii) and Cm(iii) using the equations established by Kimura,66 based on work of Horrocks67.
| EF–Eu(iii) 1 : 1 complex | EF–Tb(iii) 1 : 1 complex | EF–Cm(iii) 1 : 1 complex | ||||
|---|---|---|---|---|---|---|
| K D (μM) | τ (μs)/qwater | K D (μM) | τ (μs) | K D (μM) | τ (μs)/qwater | |
| EF1 | 5.50 ± 0.57 | 186.2 ± 3.7/5.1 | 8.47 ± 0.325 | 450 ± 18.0 | 2.28 ± 0.104 | 143.1 ± 19/3.7 |
| EF2 | 6.52 ± 0.48 | 181.8 ± 8.6/5.3 | 8.24 ± 1.31 | 469 ± 5.30 | 2.86 ± 0.084 | 147.7 ± 18/3.5 |
| EF3 | 7.42 ± 1.04 | 182.1 ± 2.2/5.3 | 8.46 ± 0.864 | 502 ± 11.0 | 2.77 ± 0.229 | 137.4 ± 15/3.9 |
| EF4 | 7.56 ± 1.49 | 186.3 ± 4.3/5.1 | 7.85 ± 1.23 | 502 ± 4.00 | 2.34 ± 0.088 | 142.7 ± 3.0/3.7 |