Table 1.

Primer and polymerase chain reaction conditions for matrix metalloproteinase-9 gene

Gene	Primer	PCR condition
MMP-9-1562 C/T	5’ATCTCCATCTCACAGTCTCATTT 3’(forward) 5’GCCTCCCTCACTCCTTTCTT3’ (reverse)	96°C for 5 min, 30 cycles (at 96°C for 30 s each), 58.5°C for 2 min, 72°C for 1 min, final elongation at 72°C for 7 min

MMP-9 – Matrix metalloproteinase-9; PCR – Polymerase chain reaction; °C – Degree celsius; min – Minutes; sec – Seconds; C/T – Cytosine/thymine