Fig 3. The identified H-NS binding Sites II and III are important for regulation of yscW-lcrF promoter activity.

(A) Schematic of PyscW-lcrF::lacZ fusions. Five constructs (p1-p5) were used to assess which regions of pyscW-lcrF allows for H-NS-YmoA repression and IscR activation. (B) Yersinia harboring the various pyscW-lcrF::lacZ plasmids were grown aerobically under T3SS-inducing conditions (low calcium LB at 37°C) for 1.5 hrs and assayed for β-galactosidase (Miller units). The average of at least three biological replicates are shown ± standard deviation. Statistical analysis was performed using an unpaired Student’s t-test (*p < .05, **p < .01, ***p < .001, ****p < .0001, and n.s. non-significant).