Fig 5. IscR is dispensable for type III secretion in the ΔymoA mutant background.

Yersinia strains were grown aerobically under T3SS-inducing conditions (low calcium at 37°C). (A) Precipitated secreted proteins were visualized by SDS-PAGE followed by Coomassie blue staining. Bovine serum albumin (BSA) was used as a loading control (left panel). Densitometry was used to measure the relative amount of secreted YopE T3SS effector protein versus BSA control. The average of four independent replicates ± standard deviation is shown (right panel). (B) RNA was extracted and RT-qPCR was used to measure relative levels of lcrF mRNA normalized to 16S rRNA. The average of at least three biological replicates are shown ± standard deviation. (C) LcrF protein levels were determined by Western blotting (left panel) and densitometry (right panel) relative to the RpoA loading control. Shown is the average of four independent replicates ± standard deviation. Statistical analysis was performed using an unpaired Student’s t-test (*p < .05, **p < .01, ***p < .001, and n.s. non-significant).