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. 2022 Jul 28;18(7):e1010321. doi: 10.1371/journal.pgen.1010321

Fig 6. Oxygen-dependent control of lcrF requires YmoA.

Fig 6

Yersinia strains were cultured under T3SS inducing conditions under aerobic (black bars) or anaerobic (grey bars) conditions. Levels of lcrF (A) and iscR (B) mRNA levels were measured by RT-qPCR and normalized to 16S rRNA. The average of at three biological replicates are shown ± standard deviation. (C) Yersinia strains were grown under similar conditions as stated above and whole cell extracts were probed for RpoA, IscR, H-NS, LcrF, YopE, and YmoA by Western blotting. One representative experiment out of three biological replicates is shown. Statistical analysis was performed using a one-way ANOVA with Tukey multiple comparisons (*p < .05,**p < .01,****p < .0001, and n.s. non-significant).