Fig 4. Deletion of one of the four S. pombe genes encoding tRNA_i^Met(CAU) in an S. pombe trm6Δ mutant exacerbates its growth defect and further reduces tRNA_i^Met(CAU) levels.

(A) Deletion of the imt06⁺ gene encoding tRNA_i^Met(CAU) in an S. pombe trm6Δ mutant severely exacerbates its growth. Strains were grown overnight in YES media at 30°C and analyzed for growth as in Fig 1A on indicated plates and temperatures for 4 days. (B) Quantification of tRNA_i^Met(CAU) levels in S. pombe trm6Δ imt06Δ mutants at 30°C. tRNA levels were quantified as in Fig 2B.