Fig 6. The temperature sensitivity and reduced tRNAiMet(CAU) levels in S. cerevisiae trm6-504 mutants are suppressed by a met22Δ mutation.
(A) A met22Δ mutation substantially suppresses the S. cerevisiae trm6-504 temperature sensitivity. Strains were grown overnight in YPD media at 30°C and analyzed for growth on indicated plates and temperatures. BY; standard BY4741 WT strain background; Y190, background of original trm6-504 mutant (B) A met22Δ mutation substantially restores tRNAiMet(CAU) levels in S. cerevisiae trm6-504 mutants. Strains were grown in YPD media at 27°C and shifted to 34°C for 8 hours as described in Materials and Methods, and RNA was isolated and analyzed by northern blotting. (C) Quantification of northern analysis of tRNAiMet(CAU) levels in S. cerevisiae BY trm6-504 mutants. tRNA levels were quantified as in Fig 2B. m, trm6-504 mutant.