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. 2022 Aug 9;13:4671. doi: 10.1038/s41467-022-32250-y

Fig. 1. Schematic of the PCR-LwCas13a assay for detection and genotyping of Treponema pallidum.

Fig. 1

The target is pre-amplified by PCR with DNA as the input. PCR products are transferred to and detected in a reaction mixture containing T7 RNA polymerase, LwCas13a, target-specific crRNA, and an RNA reporter that fluoresces at Ex/Em = 490 nm/520 nm when cleaved. Three targets of TPA were detected in separate reactions in triplicate for diagnosis, identification of lineage and macrolide resistance genetic markers, respectively.