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. 2022 Aug 9;13:4671. doi: 10.1038/s41467-022-32250-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The PCR-LwCas13a assay exhibits excellent detection of the tpp47 gene and is an order of magnitude more sensitive than TaqMan PCR. b Evaluation of the specificity of the PCR-LwCas13a assay by testing DNA from a group of 10 genital microorganisms in 180 min of kinetics analysis. c Samples from 135 syphilis and 81 non-syphilis patients (216 total) were used to determine the clinical sensitivity and specificity of the PCR-LwCas13a assay in a parallel comparison with TaqMan PCR. Rabbit-infectivity testing (RIT) was performed on 9 chancre exudates and 32 whole blood samples from secondary syphilis. d Performance of the PCR-LwCas13a assay and TaqMan PCR with clinical specimens. e Comparison of direct detection with whole blood from 32 patients with secondary syphilis and chancre exudates from nine patients with primary syphilis. Squares with color (orange for PCR-LwCas13a assay, blue for rabbit-infectivity test, and red for TaqMan PCR) represent positive signals, while white squares represent negative results. Darkfield microscopy of testicular extracts was used to determine RIT results. f Comparison of the sensitivities of direct detection methods. Abbreviations: A.U. Arbitrary units, PS Primary syphilis, SS Secondary syphilis, CE Chancre exudate, WB Whole blood, RIT rabbit-infectivity test. n = 3 technical replicates; two-tailed Student t-test was used to analyze the statistical significance; p-value was labeled in the figure, ns = not significant; error bars represent mean ± SEM. Source data are provided as a Source Data file.