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. 2022 Aug 9;13:4671. doi: 10.1038/s41467-022-32250-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic of PCR-LwCas13a assay for TPA macrolide resistance genotyping. b Dilution experiment to assess the limits of detection of 23 S rRNA mutation (A2058G). c Evaluation of the specificity of PCR-LwCas13a assay for 23 S rRNA mutations by testing a panel of DNAs from 10 genital microorganisms. d Ten clinical samples were evaluated by PCR-LwCas13a assay and validated by Sanger sequencing. Squares with color represent positive signals, while white squares represent negative results. Abbreviations: A.U. Arbitrary units. n = 3 technical replicates; two-tailed Student t-test was used to analyze the statistical significance; p value was labeled in the figure, ns = not significant, error bars represent mean ± SEM. Source data are provided as a Source Data file.