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. 2022 Aug 9;13:4677. doi: 10.1038/s41467-022-32149-8

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Mice were vaccinated I.D. with a single dose of 5 μg of NP, NA, HA or control luciferase mRNA-LNPs. Splenocytes collected from immunized animals 10 days after immunization were stimulated with NP or NA or HA overlapping peptide pools, and cytokine production by CD4⁺ and CD8⁺ T cells was assessed by flow cytometry (a). Percentages of B/Col NP- (p-values left to right 0.0023, <0.0001, 0.028, <0.0001, 0.0004, 0.0478) (b), B/Col NA- (p-values left to right <0.0001, <0.0001, 0.0014, <0.0001, 0.0001, 0.2702) (c), B/Col HA- (p-values left to right 0.1794, 0.0002, 0.0047, 0.0037, 0.0023, 0.1322) (d) and B/Phu HA- (p-values left to right 0.026, <0.0001, 0.0031, 0.0006, 0.0003, 0.5177) (e) specific CD4⁺ and CD8⁺ T cells producing IFN-γ, IL-2 and TNF-α are shown. Each symbol represents one animal and error is shown as SEM (n = 8 mice per group for B/Col NP and n = 7 for B/Col NA, B/Col HA, B/Phu HA and luciferase). Statistical analysis: two-tailed unpaired t-test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗p < 0.0001. Source data are provided as a source data file.