TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| P. fluorescens | ||
| Pf-5 | Rhizosphere isolate; Plt+, Ampr | 25 |
| JL4365 | pltE::Tn3-nice derivative of Pf-5; Plt−, Ina+, Kmr | 30 |
| JL4389 | pltB::Tn3-nice derivative of Pf-5; Plt−, Ina+, Kmr | 30 |
| JL4390 | pltF::Tn3-nice derivative of Pf-5; Plt−, Ina+, Kmr | 30 |
| JL4562 | pltM::aphI derivative of Pf-5, constructed by marker exchange with pJEL6049; Plt−, Tcr, Kmr | This study |
| JL4563 | pltR::aacC1 derivative of Pf-5; Plt−, Gmr | This study |
| JL4564 | pltR::aacC1, pltE::Tn3-nice derivative of Pf-5, constructed by marker exchange mutagenesis of JL4365 with pJEL6051; Plt−, Gmr, Kmr | This study |
| JL4565 | Pf-5 with pltR::aacC1, pltB::Tn3-nice derivative of Pf-5, constructed by marker exchange mutagenesis of JL4389 with pJEL6051; Plt−, Gmr, Kmr | This study |
| JL4566 | pltR::aacC1, pltF::Tn3-nice derivative of Pf-5, constructed by marker exchange mutagenesis of JL4390 with pJEL6051; Plt−, Gmr, Kmr | This study |
| E. coli | ||
| DH5α | F−endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA96, relA1, 80dlacZ, M15, λ− | 44 |
| S17-1 | Res−, Mod+, recA, Tra+ | 50 |
| Plasmids | ||
| pLAFR3 | IncP1 replicon; cos, Mob+, Tcr | 51 |
| pME3087 | ColE1 replicon; Mob+, Tcr | 59 |
| pMGm | ColE1 replicon, source of aacC1 cassette; Ampr, Gmr | 36 |
| pRK415 | IncP1 replicon; Mob+, Tcr | 27 |
| pUC4K | ColE1 replicon, source of aphI cassette; Ampr, Kmr | 58 |
| pUC18, pUC19 | ColE1 replicon; Ampr | 44 |
| pUC18Δ EcoRI | pUC18 with the EcoRI site removed from the polylinker; Ampr | 8 |
| pJEL1938 | 29 kb of genomic DNA from Pf-5 containing part of the plt region cloned into pLAFR3; Tcr | 30 |
| pJEL1939 | ca. 24 kb of genomic DNA from Pf-5 containing part of plt region cloned into pLAFR3; Tcr | 30 |
| pJEL5981 | 2.2-kb KpnI-HindIII fragment from pJEL1938 containing pltR of Pf-5 cloned in pUC18ΔEcoRI; Ampr | This study |
| pJEL6041 | 2.7-kb EcoRI fragment from pJEL1939 containing pltM cloned into pUC19; Ampr | This study |
| pJEL6043 | pltR::aacC1, constructed by cloning a 2.0-kb BamHI fragment containing aacC1 from pMGm into the EcoRI site internal to the pltR gene in pJEL5981; Ampr, Gmr | This study |
| pJEL6047 | pltM::aphI, constructed by cloning an AccI fragment containing aphI from pUC4K into the NspV site internal to pltM in pJEL6041; Ampr, Gmr | This study |
| pJEL6049 | pltM::aphI, constructed by cloning 4-kb EcoRI fragment containing pltM::aphI from pJEL6047 into pME3087; Tcr, Gmr | This study |
| pJEL6051 | pltR::aacC1, constructed by cloning the 4.4-kb HindIII-KpnI fragment containing pltR::aacC1 from pJEL6043 into pRK415; Tcr, Gmr | This study |