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RsaL’s repressive effect is overcome by addition of exogenous PAI-1. β-Galactosidase activity from a plasmid-borne lasB-lacZ translational fusion was monitored in the presence of either lasR alone or both lasR and rsaL in P. aeruginosa PAO-R1 after growth in either the presence or absence of 1 μM exogenous PAI-1. Expression of lasR and rsaL was directed by either their native promoters (wt) or the lac promoter (plac) as indicated under each plasmid.
