TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant phenotype of characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| PAO1 | Prototrophic | 13 |
| PAO-JP1 | ΔlasI::tet, LasI null mutant | 27 |
| PAO-R1 | Smr ΔlasR::tet, LasR null mutant | 9 |
| PDO111 | rhlR::Tn501 derivative of PAO1, Hgr, RhlR null mutant | 4 |
| E. coli | ||
| MG4 λI14 | Δ(argF-lac)U169 zah-735::Tn10 recA56 srl::Tn10 lasI::lacZ lysogen | 35 |
| MG4 λB2 | Δ(argF-lac)U169 zah-735::Tn10 recA56 srl::Tn10 lasB::lacZ lysogen | 35 |
| Plasmids | ||
| pEX1.8 | Ampr, stabilizing fragment (1.8-kb PstI fragment) | 27 |
| pEXR | AmprlasRpwt | This study |
| pEXRL | AmprrsaLptac | This study |
| pEXRR | AmprrsaLptac (800-bp SalI-HphI fragment) lasRpwt | This study |
| pEXRR2 | AmprrsaLptac (307-bp SfiI-HphI fragment) lasRpwt | This study |
| pEXRR/NS | AmprrsaLptac (start methionine ATG to CTG) lasRpwt | This study |
| pPCS2001 | AmprlasI-lacZ | This study |
| pPCS2002 | AmprrsaL-lacZ | This study |
| pKDT17 | AmprlasB-lacZ lasRplac | 25 |
| pPCS15 | AmprlasB-lacZ lasRplac rsaLpwt | This study |
| pTS4001.7 | AmprlasB-lacZ lasRpwtrsaLpwt | 23 |
| pPCS16 | AmprlasB-lacZ rsaLplac lasRpwt | This study |
| pPCS15/rsaL-lacZ | AmprlasRplac rsaLpwt-lacZ | This study |
| pTS4001.7/rsaL-lacZ | AmprlasRpwtrsaLpwt-lacZ | This study |
| pPCS16/rsaL-lacZ | AmprlasRpwtrsaLplac-lacZ | This study |
| pPCS16/ES | AmprlasB-lacZ rsaLplac (codon 3 TCA to TAA) lasRpwt | This study |
| pPCS16/NS | AmprlasB-lacZ rsaLplac (start methionine ATG to CTG) lasRpwt | This study |
| pTS400 | Ampr, lasB-lacZ translational fusion on pSW205 | 23 |
| pSW205 | AmprlacZ′, stabilizing fragment (1.8-kb PstI fragment) | 10 |
| pSWRL | AmprrsaL-lacZ (out-of-frame translational fusion) | This study |
| pSWRL2 | AmprrsaL-lacZ (in-frame translational fusion) | This study |
| pTrcHis | Ampr, A, B and C vectors for cloning in all three frames | Invitrogen |