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LY294402 blocked the function of RACGAP1. Clone formation (a), transwell (b) and CCK-8 (c) after cotransfection with vector or RACGAP1 and DMSO or LY294002 in Huh7 and HCCLM3 cell lines. (d) The expression of core markers in PI3K/AKT signaling pathway after cotransfection with vector or RACGAP1 and DMSO or LY294002 by western-blotting in Huh7 and HCCLM3 cell lines. ∗p <0.05; ∗∗p <0.01; ∗∗∗p <0.001.
