FIG. 2.

Effects of suppressor mutations on kinetics of PAI2-AP transcription upon induction with arabinose. Cells grown to an A₆₀₀ of 0.1 in NZ medium at 37°C were induced with 0.2% arabinose at time zero. Total RNA was extracted from samples collected at the indicated times. Two micrograms of RNA was hybridized to a ³²P-labeled PAI2 cRNA probe (290 nt). After digestion with pancreatic RNase, the hybrids were denatured and electrophoresed in a 6% urea-polyacrylamide gel. The protected cRNA fragment is 193 nt long (arrow); traces of undigested probe are visible in the upper portion of the gel. Lanes 1 to 5, parental strain SB0; lanes 6 to 10, suppressor strain SB1; lanes 11 to 15, suppressor strain SB34.