FIG. 3.

Nucleotide sequences of mutations in the ydeA promoter. The nucleotide sequence of the wild-type (wt) ydeA promoter region is shown at the top; numbering corresponds to that of database entry ECAE000250. The putative −35 and −10 promoter regions are boxed, and the potential start sites are underlined. The mutation in strain SB34 introduces a G residue in the transcription initiation region. The large deletion in strain SB1 changes the −35 region, which now shows a five-of-six-position match with the TTGACA consensus sequence; the insert of pSB11 starts at the underlined Sau3AI site. The sequence upstream of the deletion is in italics and boxed, as is the wild-type sequence at the beginning of terC, shown at the bottom; numbering corresponds to that of database entry ECAE000249. The ACAAAT repeats (thick lines) upstream of terC (bottom) and ydeA (top) may have been involved in the spontaneous deletion of intervening sequences.