Fig. 1.

Scheme of culture conditions designed to study the production of lentiviral vector using clone P/cSIN/92. The clone was first cultivated in HyCell TransFx-H to examine volumetric virus productivity in batch cultures induced at cell density of 1, 2, 3 and 4E6 cells/mL and without medium exchange. The cultures grown to different concentrations were also taken out, centrifuged and then resuspended to fresh media at 1E6 cells/mL (medium exchange & dilution) before induction to assess specific virus productivity of cell. The clone was also, respectively, grown in three culture media, HyCell TransFx-H, HEK TF and a mixture of 50% HEK TF and 50% HyCell TransFx-H, and fed with Cellboost 5 or HEK FS feed to a cell density of 5E6 cells/mL and induced with or without medium exchange prior to induction. The induced culture was then fed at 1 and/or 24 hpi as needed by experimental design to investigate the virus production in fed-batch culture induced at high cell density (5E6 cells/mL)