Fig. 8.

a Lentiviral vector production in clone P/cSIN/92 cultures grown in HEK TF, HyCell TransFx-H (HyCell), or a mixture of HyCell TransFx-H and HEK TF, and fed with HEK FS feed to a cell density of 5E6 cells/mL and induced with or without medium exchange to fresh HyCell TransFx-H. Two cultures (HEK TF and HyCell, 1E6 cells/mL w/o M/E) grown in HEKTF and HyCell TransFx-H (HyCell), respectively, to a cell density of 1E6 cells/mL and induced without medium exchange were conducted as references. b Cell-specific vector productivity of clone P/cSIN/92 taken from cultures grown in HEK TF, HyCell TransFx-H (HyCell), or a mixture of HyCell TransFx-H and HEK TF to cell density of 1E6 and 5E6 cells/mL (with HEK FS feed), then resuspended to fresh HyCell TransFx-H at 1E6 cells/mL prior to induction