Table 2.
A summary of typical results of volumetric LV productivity of clone P/cSIN/92 grown in HyCell™ TransFx-H culture, fed with CB5 and/or other nutrients and with or without medium exchange before induction
| Cell density at induction (cells/mL) | Medium exchange | Feed | Titer (TU/mL) | SD (TU/mL) |
|---|---|---|---|---|
| 1 × 106 | No | – | 1.53E + 07 | 1.2E + 06 |
| 3 × 106 | No | 3% cell boost 5 | 1.28E + 07 | 1.5E + 06 |
| 5 × 106 | No | – | 1.09E + 07 | 5.0E + 05 |
| 5 × 106 | No | 50 µg/mL insulin | 1.40E + 07 | 1.5E + 06 |
| 5 × 106 | No | 2% cell boost 5 + 2% yeast extract | 1.85E + 07 | 2.0E + 06 |
| 5 × 106 | No | 5% cell boost 5 | 8.0E + 06 | 8.0E + 05 |
| 5 × 106 | Yes | – | 3.38E + 07 | 2.0E + 06 |
SD standard deviation from duplicate infected cultures