FIG. 5.

Southern blot analysis of the mitA mutant strain MV100. (A) Construction of mitA disruption mutant and restriction map of the wild type and mitA disruption mutant showing expected band sizes in the Southern blot. The maps are not drawn to scale. (B) S. lavendulae genomic DNAs from the wild type (lanes 1 and 2) and the double-crossover mutant MV100 (lanes 3 and 4) were digested with BamHI (lanes 1 and 3) and SphI (lanes 2 and 4), respectively. The 4.9-kb EcoRI-HindIII fragment from pDHS2001 containing tsr-disrupted mitA was used as the probe.