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. 2022 Aug 9;13:4661. doi: 10.1038/s41467-022-32346-5

Fig. 6. Activation of NF-κB pathway in RAW-Blue cells and macrophage polarization in THP-1 cells.

Fig. 6

a Dose-dependent NF-κB/AP-1 response elicited by PASs polymers, β-glucans, and dextran: 37 °C, 24 h. (N = 2 independent experiments, n = 2 per condition). b Effect of anti-Dectin-1 blocking antibody, or an isotype control antibody (10 µg/mL) on the NF-κB/AP-1 activation induced by P5, P7, and Lam: 100 μg/mL, 37 °C, 24 h. Two-tailed unpaired t test were performed: Lent: Isotype vs Anti-Dectin (p < 0.0001), Lam: Isotype vs Anti-Dectin (p < 0.0001), P7: Isotype vs Anti-Dectin (p < 0.0001). c M1 and d M2 macrophage-related marker mRNA expression in polymer-treated THP-1 cells: 100 μg/mL, 37 °C, 24 h. (N = 3 independent experiments, n = 1 per condition). Two-tailed unpaired t test were performed for M1 macrophage-related mRNA expression: TNF: Ctrl vs P7 (p < 0.0001), Ctrl vs Lam (p = 0.0001), IL1B: Ctrl vs P7 (p < 0.0001), Ctrl vs Lam (p < 0.0001), IL6: Ctrl vs P7 (p < 0.0001), Ctrl vs Lam (p < 0.0001), IL12A: Ctrl vs P7 (p < 0.0001), MMP9: Ctrl vs P7 (p = 0.0026), Ctrl vs Lam (p = 0.001), CD68: Ctrl vs P7 (p = 0.0002), Ctrl vs Lam (p = 0.006), CD86: Ctrl vs P7 (p < 0.0001), Ctrl vs Lam (p = 0.0165), NOS2: Ctrl vs P7 (p = 0.0003), Ctrl vs Lam (p = 0.0152). Unless otherwise stated, data are means ± SD of three independent experiments (N = 3), triplicates (n = 3) per condition were used. ns p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 compared to control using two-tailed unpaired t test. Abbreviations used: Ctrl = Control, Dex = Dextran, Lent = Lentinan, Lam = Laminarin.