Fig. 2. MYC drives nucleotide production through the salvage pathway in the absence of glutamine and causes reversal of the non-oxidative pentose phosphate pathway.

A, B MRC-5 cells expressing MYC-ER^T2 (MYC) or vector control (VEC) were labelled with (A) ¹⁵N-glycine for 12 h or (B) ¹³C₆-glucose for 6 h and the indicated metabolites and isotopologues were measured by LC-MS. An ordinary one-way ANOVA was used to determine statistical significance (n = 6 biological replicates). C–F Cells were treated with 6-AN at the doses shown (in μM), and a 5 min pulse with ¹³C₆-glucose was used to visualise flux into (C) 6-phosphogluconate, (D) glyceraldehyde 3-phosphate, (E) pyruvate, and (F) ribose 5-phosphate. G Scheme showing how MYC reroutes glycolytic carbon to support nucleotide biosynthesis. For all statistical tests P ≤ 0.05 was considered significant, and error bars show the standard error of the mean.