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. 2022 Aug 9;5:798. doi: 10.1038/s42003-022-03768-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Overlay of ¹H-¹⁵N HSQC spectra of 0.3 mM ¹⁵N-enriched FL- and CT-α-syn in the absence (black) and presence (red) of 0.15 mM PDI. Perturbed residues are surrounded by a dotted box. b Residue-resolved signal attenuation (I/I₀) of 0.3 mM FL- (black circles) and CT-α-syn (red circles) upon adding 0.15 mM PDI. Values <1.0 indicate interactions. Binding regions are colored gray. c Representative ¹H-¹⁵N HSQC cross-peaks within segments 1 and 2 of 0.3 mM FL- and CT-α-syn titrated with PDI. d Average CSPs of distinguishable residues within segment 1 (V3, M5, G7, L8, S9, K12, V15, A17, A18, A19, and E20) and 2 (T33, V37, L38, V40, G41, and T44) of FL- and CT-α-syn as a function of the PDI concentration. K_D values from curve fitting are shown. CSP values of FL- and CT-α-syn at 0.6 mM PDI were predicted by fitting the curve due to signal attenuation from segments 1 and 2 at higher (>0.3 mM) PDI concentration.