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. Author manuscript; available in PMC: 2023 Nov 18.
Published in final edited form as: ACS Chem Biol. 2022 Feb 10;17(11):3047–3058. doi: 10.1021/acschembio.1c00906

Figure 1.

Figure 1.

Qβ is a powerful carrier to generate anti-Tn IgG antibody responses. (a) Synthesis of NHS Tn 1 and its conjugation with Qβ. (b) Mice were immunized with Qβ-Tn (1.9 μg of Tn per injection) and Qβ on days 0, 14, and 28. Blood was collected on days −1 and 35. (c) The titers of anti-Tn IgG antibodies in the sera on day 35 were analyzed from immunized mice by ELISA against the BSA-Tn conjugate. Preimmune refers to blood collected on day −1. Statistical analysis was performed using the Student t test by GraphPad Prism. ****p < 0.0001.