Fig. 4.
Regulatory effect of miR-379-5p on HMGCS1. a The mRNA expression of cholesterol metabolism-related genes (SREBF2, HMGCR, HMGCS1, SCARB1, CEH, Cyp7a1, Cyp27a1, ABCA1 and ABCG1) in the livers of HFHC mice, n = 7–8 in each group. b HMGCS1 protein levels in the livers of HFHC mice, n = 7–8 in each group. c FC contents in Huh7 cells that were transfected with miR-379-5p mimics (30 nM) or siHMGCS1 (30 nM) for 24 h and then cultured with 0.5 mM PA for another 24 h. d & e HMGCS1 protein and mRNA levels in Huh7 cells transfected with miR-379-5p mimics (30 nM) or NC (30 nM) for 48 h and then cultured with 0.5 mM PA for another 24 h. f The relative activities of HMGCS1 3’UTR presented by relative luciferase activity of HEK293 cells co-transfected with related plasmids and miR-379-5p mimics (30 nM) or NC(30 nM) for 24 h. For a, c, e-f, two-tailed student’s t-test was used to calculate statistical significance, while for b, One-Way ANOVA test was used. *p < 0.05, **p < 0.01, ***p < 0.001, n = 3 independent experiments