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. 2022 Feb 10;29(8-9):1240–1251. doi: 10.1038/s41417-022-00431-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Structure of the transgene-carrying oFV vectors used in the study.TK – HSV-1 thymidine kinase, iCasp9—inducible caspase 9, T2A—T2A self-cleaving peptide. B indicator U251-U3-mCherry-U3-Luc cells express firefly luciferase only in response to FV infection. Wild type U251, U251-U3-mCherry (expressing mCherry under the FV U3 promoter), U251-U3-mCherry-SSFV-Luc (expressing mCherry under the FV U3 promoter and firefly luciferase under the constitutive SFFV promoter), U251-U3-mCherry-U3-Luc (expressing both mCherry and firefly luciferase under the FV U3 promoter) were infected with oFV at indicated MOIs. 3 days postinfection the luciferase assay was performed to determine the luciferase expression in the infected cells. The experiment was performed in duplicate in two independent experiments. U3—FV promoter-containing region from the viral LTR, Luc- firefly luciferase. C. Subcutaneous indicator U251-U3-mCherry-U3-Luc tumors express firefly luciferase in response to oFV infection and the bioluminescence was imaged using IVIS Xenogen. Tumors were directly injected with 2 doses of 1 * 10⁶ IU of the parental oFV or PBS control and imaged 29 days postinfection.