Figure 1.

Membrane cholesterol paradoxically inhibits PLY-induced calcium-entry. Membrane cholesterol was either depleted (MβCD) or loaded (MβCD+Chol.) into the plasma membrane of COS7 cells for 30 minutes, followed by a 10 minutes treatment with vehicle (Veh) or PLY (60 ng/mL) and changes of intracellular calcium levels were measured by aequorin-mediated luminescence as described in materials and methods. Data are represented as mean ± SE, n.s., not significant, *P<0.05, ****P<0.0001 (n=4). One-way ANOVA (with Tukey’s post-hoc test).