Figure 2.
Caveolin-1 overexpression protects against Pneumolysin (PLY) induced calcium entry and endothelial barrier dysfunction. (A) Caveolin-1 overexpression or Cav-1 peptide blocks PLY induced Ca2+ entry in Cos7 cells. Data are represented as mean ± SE (n=3-4), * P<0.05, **P<0.01 vs. vehicle control. (B) HLMVEC cells were transduced with 10-10 MOI RFP (RFP ad.) or caveolin-1 adenovirus (Cav-1 ad.) (Inset: western blot analysis of Caveolin-1 overexpression. Hsp90 was used as loading control), or (C) treated with 10 μM caveolin-1 scaffolding domain peptide for 24 hours, and TER of vehicle or PLY (60ng/mL) treated HLMVECs was measured (n=3). ***P<0.001, ****P<0.0001. One-way ANOVA (with Tukey’s post-hoc test).