Figure 7.

(Color online) Effect of lanthanum chloride on the PI3K-AKT-HIF-1α pathway related proteins in H-vascular smooth muscle cells (VSMCs). (A) Expression levels of PI3K, p-PI3K, AKT, p-AKT, m-TOR, p-mTOR, HIF-1α, SM22α, BMP2, and VEGF in the cytoplasm of H-VSMCs. (B) Quantification of PI3K, p-PI3K, AKT, p-AKT, m-TOR, p-mTOR, HIF-1α, VEGF, BMP-2, and SM22α expression levels in the cytoplasm of H-VSMCs. (C) Expression levels of HIF-1α and RUNX2 in the nucleus of VSMCs. (D) Quantification of HIF-1α and RUNX2 expression levels in the nucleus. (E) Effect of lanthanum ion on the expression levels of VEGF, SM22α, BMP-2, and RUNX2 mRNA ( $\overline{\text{X}}\pm \text{S}$ , compared with the control group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001; compared with the model group, *p < 0.05, **p < 0.01, ***p < 0.001, n = 3).