TABLE 1.
Strains of S. meliloti and E. coli and plasmids used in this study
| Strain or plasmid | Relevant genotype or characteristica | Reference or source |
|---|---|---|
| S. meliloti strains | ||
| 104A14 | Wild type | 47 |
| RmMSU4 | 104A14 ackA::Tn5B22 | 52 |
| RmMSU5 | 104A14 pta::Ω ackA::Tn5B22 | This study |
| RmMSU6 | 104A14 pta::Ω | This study |
| RmMSU7 | 104A14 phoB::Ω ackA::Tn5B22 | This study |
| E. coli strains | ||
| S17-1 | Pro− Mob+; donor strain in matings | 43 |
| DH5α | Cloning host | 41 |
| Plasmids | ||
| pUC19 | Cloning vector; Ampr | 58 |
| pBluescript KS(+) | Cloning and sequencing vector; Ampr | Stratagene |
| pJQ200KS+ | sacB sacR Gentr SucS | 39 |
| pCPP30 | Broad-host-range plasmid; Gentr | D. Bauer, Cornell University |
| pRL453 | pUC18/19 containing L.EHE1 and C.S3; Ω fragment | 18 |
| p7C9 | Cosmid library clone containing the S. meliloti orfA-pta-ackA-fabI operon | This study |
| pMLS3 | pCPP30 with 2.4-kb XmaI-SacI p7C9 fragment; Gentr | This study |
| pMLS4 | pCPP30 with 3.2-kb BamHI-SacI p7C9 fragment; Gentr | This study |
| pMLS5 | pCPP30 with 4.0-kb BamHI p7C9 fragment; Gentr | This study |
| pMLS6 | pCPP30 with 8.8-kb NotI p7C9 fragment; Gentr | This study |
| pMLS7 | pCPP30 with 5.0-kb EcoRI-SacI p7C9 fragment; Gentr Pta+ | This study |
| pMLS8 | pCPP30 with 9-kb SacI p7C9 fragment; Gentr Pta+ | This study |
| pMLS90 | pJQ200KS+ ligated to linearized pUC19 containing the Ω-interrupted phoB in a 2.3-kb HindIII fragment | 2; this study |
| pMLS101 | pJQ200KS+ containing the linearized 9-kb SacI p7C9 fragment ligated to pBluescript KS(+); pta::Ω | This study |
a
Ampr, ampicillin resistant; Gentr, gentamicin resistant; SucS, sucrose sensitive.