FIG. 3.

2dGlc is not a morphogen. At 3 h after treatment, 1 ml of each culture prepared as described in the legend to Fig. 2 was concentrated by low-speed centrifugation and suspended in 0.1 ml of CTPM medium. Aliquots were examined by using differential interference contrast optics and a 40× objective lens with a Nikon Microphot-FXA microscope (bottom). (A) Untreated cells; B, cells plus 0.5 M glycerol; C, cells plus 1% 2dGlc; D cells plus 2% GlcNAc. Photomicrographs of cells are at the same magnification; the average length of untreated cells is ca. 10 μm.