FIG. 7.

Sporulation is inhibited by 2dGlc throughout the entire developmental cycle. Wild-type cells were grown to exponential density in rich medium, concentrated, spotted on nitrocellulose filters placed on the surfaces of plates with starvation agar, and incubated at 32°C. At the indicated times after the initiation of development, filters were removed from TPM plates and placed onto TPM plates supplemented with 0.1% 2dGlc, and incubation was continued at 32°C. Development on TPM buffer and then on TPM buffer with 2dGlc was allowed to proceed for a total duration of 120 h; then filters were heat treated and assayed for the presence of spores as described in Materials and Methods. Closed circles, spore titers of developing cells transferred from TPM agar to TPM agar with 2dGlc; open circles, spore titers of developing cells maintained on TPM agar.