FIG. 8.

Competitive inhibition of M. xanthus hexokinase by 2dGlc. Extracts (100 μl) made from M. xanthus wild-type strain DK1622 were incubated in 50 mM Tris–10 mM MgCl₂ (pH 9.1) with Glc (160 μM [squares], 200 μM [open circles], or 300 μM [filled circles]), ATP (10 mM), 500 to 1,000 U of Glc6P dehydrogenase, and Glc6P at concentrations of 0, 1.2, 2.0, 3.0, and 4.6 mM. Results from a representative experiment are plotted as 1/V (absorbance at 340 nm × 10⁵ min⁻¹)⁻¹ versus [I], the concentration (millimolar) of the inhibitor 2dGlc. Plots are linear least-square fits to the data.