TABLE 1.
Hexoseb | Efficiency of platingc
|
||
---|---|---|---|
DK1622 | DK1622::pAY703 | DK1622::pAY1108 | |
None | 1.0 | 1.0 | 1.0 |
2% Glc | 0.6 | 0.5 | 0.5 |
1% 2dGlc | 3 × 10−6 | 3 × 10−6 | <10−8 |
2% MeGlc | 0.9 | 0.9 | 0.8 |
2% GlcN | <10−8 | <10−8 | <10−8 |
2% GlcNAc | 0.6 | 0.6 | 0.4 |
2% Fru | 0.5 | 0.6 | 0.7 |
2% Mtl | 0.8 | 0.6 | 0.6 |
Wild-type strain DK1622 and its derivatives with plasmids pAY703 and pAY1108 (expressing the E. coli glk gene) integrated at the mglBA locus were grown to a density of 5 × 108/ml at 32°C in CTPM medium.
Dilutions of each culture were plated on CTPM medium or medium supplemented with each of the hexose analogs. Colonies were counted after 7 days of growth at 32°C.
Calculated as the titer of each culture on a given medium divided by its titer on CTPM medium without added hexose. Numbers are the averages of at least three determinations and varied less than fivefold.