TABLE 2.
Expression of E. coli glk restores sensitivity to 2dGlc-resistant mutants of M. xanthusa
| Plasmid (genotype) | Efficiencies of platingb
|
||
|---|---|---|---|
| Wild type | hex1 | hex3 | |
| None | 3 × 10−6 | 4 | 0.9 |
| pAY703 (mglBA) | 3 × 10−6 | 1 | 0.8 |
| pAY1108 (mglBA-glk) | <10−8 | 7 × 10−5 | 2 × 10−5 |
a
Wild-type strain DK1622 and its 2dGlc-resistant hex1 and hex mutant derivatives with no integrated plasmids or with plasmids pAY703 and pAY1108, were grown to a density of 5 × 108/ml at 32°C in CTPM medium. Dilutions of each culture were plated on CTPM medium and medium supplemented with 1% 2dGlc. Colonies were counted after 7 days of growth at 32°C.
b
Calculated as the titer of each culture on CTPM medium with 1% 2dGlc divided by its titer on CTPM medium without 2dGlc. Numbers are the averages of at least two determinations and varied less than fivefold.