TABLE 3.
Glc and GlcNAc can antagonize the sensitivity of wild-type M. xanthus cells to 2dGlc
| Supplement(s)a | Efficiency of platingb |
|---|---|
| None | 1.0 |
| 0.1% 2dGlc | 3 × 10−6 |
| 0.1% 2dGlc + 2% Glc | 0.4 |
| 0.1% 2dGlc + 2% GlcNAc | 0.4 |
| 0.1% 2dGlc + 2% Fru | 2 × 10−6 |
| 0.1% 2dGlc + 2% Mtl | 2 × 10−6 |
a
Wild-type strain DK1622 was grown to a density of 5 × 108/ml at 32°C in CTPM medium. Dilutions of each culture were plated on CTPM medium or medium supplemented with each of the combinations of hexose analogs. Colonies were counted after 7 days of growth at 32°C.
b
Calculated as the titer of each culture on a given medium divided by its titer on CTPM medium without added hexoses. Numbers are the averages of at least three determinations and varied less than fivefold.