TABLE 4.

2dGlc inhibits the ability of M. xanthus to produce heat-resistant spores in response to starvation^a

Supplementb	Efficiency of sporulationc
	hex+	hex1	hex3
0.5% 2dGlc	0.00002	40	0.00001
2.0% 2dGlc	0.000001	100	0.00002
0.5% GlcN	0.02	0.05	0.1
2.0% GlcN	0.000002	0.000001	0.000004

^aWild-type strain DK1622 (hex⁺) and its mutant derivatives (hex1 and hex3) were grown to a density of 5 × 10⁸ cells/ml in CTPM medium at 32°C, concentrated 10-fold by low-speed centrifugation, and suspended in TPM buffer. 

^bSuspended cells were spotted onto starvation agar supplemented with the indicated concentrations of hexoses and incubated at 32°C for 120 h, prior to assay for heat-resistant spores, as described in Materials and Methods. 

^cPercentage of heat-resistant spores relative to titers of heat-resistant spores that arose after development on TPM agar without supplements. Values are averages of at least three determinations and varied less than fivefold. For each of the starting strains, DK1622 (wild type), XS200 (hex1), and XS201 (hex3), the titers of heat-resistant spores per 5 × 10⁸ cells starved on TPM medium were 1.9 × 10⁷ (3.8%), 2.3 × 10⁷ (4.6%), and 1.1 × 10⁷ (2.2%), respectively.