TABLE 5.
The E. coli glk gene is expressed in M. xanthus
| Straina | Relevant genotype | Glucokinase activity (U)b |
|---|---|---|
| DK1622 | hex+ | 9.2 |
| DK1622::pAY703 | hex+ | 8.2 |
| DK1622::pAY1108 | hex+ glk+ | 310 |
| XS200 | hex1 | 1.3 |
| XS200::pAY703 | hex1 | 1.4 |
| XS200::pAY1108 | hex1 glk+ | 300 |
| XS201 | hex3 | 2.8 |
| XS201::pAY703 | hex3 | 2.7 |
| XS201::pAY1108 | hex3 glk+ | 510 |
| DK1622::pAY1108*c | hex+ | 9.5 |
a
Wild-type strain DK1622 and its mutant derivatives were grown to a density of 5 × 108 cells/ml in CTPM medium at 32°C, concentrated 10-fold by low-speed centrifugation, and suspended in TPM buffer.
b
Cell extracts were prepared by sonication and centrifugation to remove cellular debris, and the supernatants were assayed for E. coli glucokinase activity as described in Materials and Methods. Activities (nanomoles minute−1 milligram−1) are the averages of three determinations and varied less than 25%.
c
Spontaneous Kms segregant of strain DK1622::pAY1108 that has lost the integrated pAY1108 (mglBA-glk) plasmid.