TABLE 6.
The phosphorylation of glucose by M. xanthus hexokinase is inhibited by 2dGlc, GlcNAc, and GlcN
a
Hexokinase activity was assayed in lysates from wild-type M. xanthus DK1622 in the presence of 10 mM ATP, 240 μM Glc, and 0.2% (wt/vol) each potential antagonist, using the Glc6P dehydrogenase-dependent coupled assay as described in Materials and Methods.
b
Activities (averages of three determinations) varied less than 15% and are expressed as fractions of the activity in the absence of added potential inhibitor.