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. 2022 Jul 27;13:957977. doi: 10.3389/fmicb.2022.957977

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Copyright © 2022 Li, Dong, Guo, Huang, Xu, Li, Yang, Yao, Feng and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Workflow of the MAIDEN system. (A) Schematic illustration of the enzymatic process of mesophilic Ago-based diagnostics for RNA viruses. (B) Schematic diagram of the procedure for the two-step operation and the one-step operation.